Coding

Part:BBa_K1137014:Design

Designed by: Nicolas Koutsoubelis, Anne Löchner   Group: iGEM13_Paris_Bettencourt   (2013-10-04)


tracRNA-CAS9


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1721
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1721
    Illegal NheI site found at 1480
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1721
    Illegal BamHI site found at 3759
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1721
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1721
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

The plasmid was ordered from Addgene and come originally from the paper below: Wenyan Jiang, David Bikard, David Cox, Feng Zhang & Luciano A Marraffini (2013): RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nature Biotechnology 31 (3), 233-239.


References